Maloetse a sa foleng a masapo le manonyeletso: Bo-rasaense baa hlalosa

Bo-rasaense ba hlalosa karolo ea protheine e itseng molokong oa lisele tse hlokolosi ho boloka masapo

Maloetse a sa foleng a masapo le manonyeletso, a kang lefu la masapo le ramatiki, a ama batho ba limilione lefats'eng ka bophara, haholo ba tsofetseng, e theola boleng ba bophelo ba bona. Ntho ea bohlokoa ho mafu ana ka bobeli ke tšebetso e fetelletseng ea lisele tse qhalang masapo tse bitsoang li-osteoclast. Li-osteoclast li thehoa ka ho khetholla mofuta o itseng oa sele ea 'mele ea ho itšireletsa mafung e bitsoang macrophage, kamora moo e fumana karolo ea eona e ncha tlhokomelong ea masapo le manonyeletso: ho roba masapo a masapo ho lumella li-osteoblast-mofuta o mong oa sele-ho lokisa le ho nchafatsa tsamaiso ea masapo .

Ka mokhoa o pharalletseng, ho na le lits'ebetso tse peli tsa lisele tse kenang phapang ena: ea pele, sengoloa-moo len Rosa la RNA (mRNA) le entsoeng ho tsoa boitsebisong ba liphatsa tsa lefutso ho DNA-ebe phetolelo-eo tlhaiso-leseling ea mRNA e khetholloang ho hlahisa liprotheine tse etsa mesebetsi e ikhethileng ka seleng. Ho tloha ka ho sibolloa ha karolo ea protheine e itseng e bitsoang RANKL ho sebopeho sa masapo, bo-rasaense ba rarolotse karolo e kholo ea selotho seo lisele tse bontšang litsela le marang-rang a phetolo a laolang moloko oa masapo. Leha ho le joalo, lits'ebetso tsa kamora ho ngolisa lisele tse amehang li ntse li lokela ho utloisisoa.

Joale, phuputsong e ncha e phatlalalitsoeng ho Biochemical and Biophysical Research Communications, borasaense ba Tokyo University of Science, Japane, ba manollotse karolo ea protheine e bitsoang Cpeb4 ts'ebetsong ena e rarahaneng. Cpeb4 ke karolo ea "cytoplasmic polyadenylation element binding (CPEB)" lelapa la liprotheine, tse tlamang ho RNA le ho laola ts'ebetso ea phetolelo le khatello, hammoho le "mekhoa e meng ea ho hlapanya" e hlahisang liprotheine tse fapaneng. Ngaka Tadayoshi Hayata, ea neng a etelletse pele phuputso eo, oa hlalosa: “Liprotheine tsa CPEB li ameha mekhoeng e fapaneng ea likokoana-hloko le maloetse, joalo ka autism, mofetše le phapang ea lisele tse khubelu tsa mali. Leha ho le joalo, mesebetsi ea bona ka khethollo ea osteoclast ha e tsejoe hantle. Ka hona, re ile ra etsa letoto la liteko ho bontša protheine e tsoang ho lelapa lena, Cpeb4, re sebelisa litso tsa sele tsa li-macrophages tsa toeba. ”

Litekong tse fapaneng tsa setso sa sele tse entsoeng, li-macrophage tsa toeba li ile tsa tsosoa le RANKL ho baka phapang ea osteoclast mme phetoho ea setso e ile ea beoa leihlo. Taba ea mantlha, bo-rasaense ba fumane hore polelo ea liphatsa tsa lefutso ea Cpeb4, mme ka lebaka leo palo ea protheine ea Cpeb4, e eketsehile nakong ea phapang ea osteoclast. Joale, ka microscopy ea immunofluorescence, ba ile ba bona liphetoho sebakeng sa Cpeb4 ka har'a lisele. Ba fumane hore Cpeb4 e tloha cytoplasm ho ea nuclei, ha e ntse e hlahisa libopeho tse ikhethileng (li-osteoclast li tloaetse ho kopana le ho etsa lisele tse nang le li-nuclei tse ngata). Sena se supa hore ts'ebetso ea Cpeb4 e amanang le phapang ea osteoclast e kanna ea etsoa kahare ho nuclei.

Ho utloisisa hore na tšusumetso ea RANKL e baka phetoho ena ea Cpeb4 joang, bo-rasaense ba ile ba "thibela" kapa ba hatella tse ling tsa liprotheine tse kenang "tlase" litseleng tsa lets'oao la li-intracellular tse bakiloeng ke ts'usumetso. Ba supile litsela tse peli ha ho hlokahala bakeng sa ts'ebetso. Leha ho le joalo, liteko tse ling li tla hlokoa ho ithuta ka botlalo ka tatellano ea liketsahalo tse etsahalang le liprotheine tsohle tse amehang.

Kamora nako, Dr Hayata le sehlopha sa hae ba bonts'itse hore Cpeb4 e hlokahala haholo bakeng sa sebopeho sa osteoclast ho sebelisoa litso tsa macrophage moo Cpeb4 e neng e felile ka mafolofolo. Lisele tsa litso tsena ha lia ka tsa arohanngoa hape ho ba li-osteoclasts.

Ha ho kopantsoe hammoho, litholoana ke lejoe le hatang ho utloisisa lisebelisoa tsa lisele tse amehang ho thehoeng ha masapo a osteoclast. Dr Hayata o re: "Phuputso ea rona e fana ka leseli la karolo ea bohlokoa ea protheine e tlamang RNA Cpeb4 e le" tšusumetso "e ntle ea phapang ea masapo. Sena se re thusa ho utloisisa hamolemo maemo a mafu a masapo le manonyeletso mme se ka kenya letsoho ntlafatsong ea maano a kalafo bakeng sa mafu a maholo a kang lefu la masapo le ramatiki. ” Re tšepa hore boemo bo tebileng ba kutloisiso ea moloko oa li-osteoclast bo tsamaisoang ke thuto ena qetellong bo tla fetolela boleng bo ntlafetseng ba bophelo ho batho ba phelang le mafu a bohloko a masapo le manonyeletso.

Mabapi le Yunivesithi ea Saense ea Tokyo
Tokyo University of Science (TUS) ke yunivesithi e tsebahalang le e hlomphuoang, ebile e le yunivesithi e kholo ka ho fetisisa e iketselitseng lipatlisiso tsa poraefete Japane, e nang le likhamphani tse 'ne bohareng ba Tokyo le mabatooa a eona le Hokkaido. E thehiloe ka 1881, univesithi e 'nile ea kenya letsoho kholisong ea Japane saenseng ka ho kenya lerato la saense ho bafuputsi, litsebi le matichere.
Ka thomo ea "Ho theha mahlale le mahlale a morao-rao bakeng sa nts'etsopele e lumellanang ea tlhaho, batho le sechaba", TUS e entse lipatlisiso tse fapaneng ho tloha saense ea mantlha ho isa ts'ebetsong. TUS e amohela katamelo e multidisciplinary ho etsa lipatlisiso 'me a etsa ho ithuta ka matla a mang a masimo a kajeno ea bohlokoa ka ho fetisisa. TUS ke meritocracy moo se molemohali saenseng e tsejoa le ho hlokomeloa. Ke eona feela univesithi e ikemetseng Japane e hlahisitseng Moputso oa Nobel le univesithi e le 'ngoe feela e ikemetseng Asia ho hlahisa ba hapileng Khau ea Nobel lebaleng la mahlale a tlhaho.

Mabapi le Moprofesa ea Kopaneng Tadayoshi Hayata oa Univesithi ea Saense ea Tokyo
Ho tloha ka 2018, Ngaka Tadayoshi Hayata e bile Moprofesa ea Kopaneng le Mofuputsi e Moholo Lefapheng la Limolek'hule tsa Limolek'hule, Lefapheng la Saense ea Meriana, Univesithing ea Saense ea Tokyo. Laboratori ea hae e shebana le metabolism ea masapo, phapang ea lisele, pharmacology ea limolek'hule, le likarolo tse ts'oanang ho utloisisa mofuta oa mafu a masapo le a kopaneng le ho fumana liphofu tsa kalafo. Dr Hayata o hokahane le Mekhatlo e 'maloa ea Majapane le American Society for Bone and Mineral Research. O phatlalalitse lingoloa tse fetang 50 tsa mantlha mme a fana ka lipuo tse fetang 150 likopanong tsa thuto. Ntle le moo, lipatlisiso tsa hae mabapi le lefu la masapo li entse likoranteng tsa Japane makhetlo a 'maloa.

Tlhahisoleseling ea lichelete
Phuputso ena e ne e tšehelitsoe ke JSPS KAKENHI [nomoro ea thuso 18K09053]; Nanken-Kyoten, TMDU (2019); Motheo oa Nakatomi; Ts'ehetso ea Patlisiso ea Astellas; Tlatsetso ea thuto ea Pfizer; Tlatsetso ea thuto ea Daiichi-Sankyo; Tlatsetso ea Boithuto ba Teijin Pharma; Tlatsetso ea thuto ea Japane ea Eli Lilly; Tlatsetso ea Otsuka Pharmaceutical Academic; Tlatsetso ea thuto ea Shionogi; Tlatsetso ea thuto ea thuto ea bongaka ea Chugai.