Teko, e ntlafalitsoeng ke baenjiniere ba biomedical le virologists ho tsoa Univesithing ea Glasgow, e fana ka liphetho tsa phallo ea morao-rao tse ts'oanang le tlhahlobo ea lapeng ea COVID-19 nakong ea metsotso e ka bang 30.
Ka pampiri e ncha e hatisitsoeng kajeno koranteng ea Nature Communications, sehlopha sa lipatlisiso se hlalosa kamoo ba ntlafalitseng tsamaiso kateng. E thehile holim'a likatleho tsa nakong e fetileng tsa tlhahlobo e potlakileng ea mafu le vaerase Univesithing, e fana ka liphetho ka nepo ea 98%.
Hepatitis C, kokoana-hloko ea mali e senyang sebete, ho hakanngoa hore e ama batho ba fetang limilione tse 70 lefatšeng ka bophara. Litlamorao tsa kokoana-hloko sebeteng li tsamaea butle, ’me bakuli ba ka ’na ba se ke ba elelloa hore ba tšoaelitsoe ho fihlela ba kula haholo ka mathata a kang lefu la ho thatafala ha sebete kapa mofetše.
Haeba tšoaetso e fumanoa pele e tsoela pele haholo, e ka phekoloa ka katleho ka meriana e theko e tlaase, e fumanehang habonolo. Leha ho le joalo, hoo e ka bang karolo ea 80 lekholong ea batho ba nang le kokoana-hloko ha ba tsebe tšoaetso ea bona ho fihlela mathata a bophelo a hlaha.
Ka lebaka leo, batho ba ka bang 400,000 lefatšeng ka bophara ba bolaoa ke mafu a amanang le lefu la sebete la C selemo se seng le se seng, bao bongata ba bona ba ka beng ba pholositsoe ke tlhahlobo le kalafo ea pejana.
Hajoale, tšoaetso ea hepatitis C e fumanoa maemong a laboratoring ho sebelisoa mehato e 'meli e lekang mali bakeng sa boteng ba li-antibodies le ho lemoha RNA kapa li-antigen tsa mantlha.
Ts'ebetso ena e ka nka nako e ngata ho fana ka liphetho, ho eketsa monyetla oa hore bakuli ba bang ba etsang tlhahlobo ba se ke ba khutlela ho ea ithuta ka sephetho. Ho fihlella litekong ho boetse ho na le moeli linaheng tse nang le chelete e tlaase le e bohareng, moo boholo ba batho ba nang le lefu la sebete la mofuta oa C ba phelang teng.
Le ha liteko tse nkehang habonolo tse khonang ho fana ka liphetho tse potlakileng li ntlafalitsoe lilemong tsa morao tjena, ho nepahala ha tsona ho ka fokotsoa, haholo-holo ho pholletsa le mefuta e fapaneng ea batho.
Sistimi e ncha ea sehlopha se etelletsoeng pele ke Univesithi ea Glasgow, leha ho le joalo, e loketse ho sebelisoa lefatšeng ka bophara. E hlophisitsoe ho tsoa ho sistimi e ts'oanang eo ba e ntlafalitseng ho fana ka tlhahlobo e potlakileng ea lefu la mala, e lekiloeng ka liphetho tse khothatsang Uganda.
Sesebelisoa sena se sebelisa maqephe a pampiri ea boka e phuthetsoeng e kang ea origami ho lokisetsa lisampole bakeng sa mokhoa o tsejoang ka hore ke loop-mediated isothermal amplification, kapa LAMP. Mokhoa oa ho phutha pampiri o nolofalletsa sampole ho sebetsoa le ho isoa likamoreng tse tharo tse nyane ka har'a khatriji, eo mochini oa LAMP o futhumatsang le ho e sebelisa ho lekola disampole bakeng sa boteng ba lefu la sebete la C RNA. Mokhoa ona o bonolo ka ho lekaneng hore o na le monyetla, nakong e tlang, oa ho tsamaisoa tšimong, ho tsoa sampuling ea mali e nkiloeng ho mokuli ka monoana.
Ts'ebetso e nka metsotso e ka bang 30. Liphetho li fanoa ka mokhoa o bonolo oa ho bala oa lateral flow joalo ka tlhahlobo ea bokhachane kapa tlhahlobo ea lapeng ea COVID-19, e bonts'ang lihlopha tse peli bakeng sa sephetho se setle le sehlopha se le seng bakeng sa negative.
E le ho hlahloba prototype ea bona, sehlopha se sebelisitse mokhoa ona ho hlahloba lisampole tsa plasma tsa mali tse sa tsejoeng tse 100 ho tsoa ho bakuli ba nang le tšoaetso e sa foleng ea HCV le mehlala e meng ea 100 ho tsoa ho bakuli ba HCV-negative, ba neng ba sebetsa e le sehlopha sa taolo. Lisampole li ile tsa lekoa ho sebelisoa tlhahlobo ea hepatitis C e tloaelehileng ea Abbott RealTime ho netefatsa liphetho tsa LAMP. Liteko tsa LAMP li fane ka sephetho se nepahetseng ka 98%.
Sehlopha se ikemiselitse ho sebelisa mokhoa ona litekong tsa masimo Afrika e ka boroa ho Sahara selemong se tlang.
Pampiri ea sehlopha, e nang le sehlooho se reng 'Loop mediated isothermal amplification e le sesebelisoa se matla sa tlhahlobo ea pele ea kokoana-hloko ea hepatitis C', e hatisitsoe ho Nature Communications. Patlisiso e ne e tsheheditswe ke ditjhelete tse tswang Lekgotleng la Dipatlisiso tsa Boenjineri le Fisikia (EPSRC), Lekgotla la Dipatlisiso tsa Bongaka le Wellcome Trust.
SEO U LOKELANG HO SE NKA HO SEHLOOHO ENA:
- The process of paper folding enables the sample to be processed and delivered to three small chambers in a cartridge, which the LAMP machine heats and uses to test the samples for the presence of hepatitis C RNA.
- The technique is sufficiently simple that it has the potential, in future, to be delivered in the field, from a blood sample taken from a patient via fingerprick.
- The results are delivered through an easy-to-read lateral flow strip like a pregnancy test or a home COVID-19 test, which shows two bands for a positive result and one band for a negative.
